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Vliv struktury krmných směsí na parametry metabolismu pomalu rostoucích kuřat
Čumplík, Lukáš
This diploma thesis deals with the influence of the structure of feed mixtures on the metabolism parameters of slow-growing broilers. The issue of this topic was documented in the literature review. In the experimental part, an experiment was described regarding the determination of the influence of the structure of feed mixtures on nitrogen retention, ileal digestibility, parameters of organs of the digestive tract, histomorphometric parameters and the structure of feces. For research purposes, 120 slow-growing Hubbard JA 57 broilers were fattened for 49 days. During the fattening period, all broilers were fed feed mixtures identical in terms of nutrients, they differed only in the structure of the feed. 3 different textures were created - fine, medium and coarse. The feed mixtures were sieved through a system of sieves to determine the proportion of particle sizes. The influence of the structure of the feed mixtures on nitrogen retention was statistically significant difference (P<0.05) in the BR3 feed mixture, when it was shown that coarse particles have a positive effect on nitrogen retention. The results were not statistically significant for the BR2 feed mixture (P>0.05). When investigating the influence of the structure of the feed mixtures on the parameters of the organs of the digestive tract, a statistically significant difference (P<0.05) was found in the height of the muscle of the gizzard. For the other investigated parameters, the results were not statistically significant (P>0.05). Regarding the histomorphometric measurement, a statistically significant difference (P<0.05) was observed between the coarse feed mixture and the ratio of the length of the villi and the depth of the crypts in the duodenum and the depth of the crypts in the ileum. At the same time, it was evaluated as a statistically significant difference (P<0.05) between the structure of the feed and the structure of the droppings.
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Digestive proteases of termites
Čermáková, Markéta ; Konvalinka, Jan (advisor) ; Ryšlavá, Helena (referee)
Digestive proteolysis in termites has not been studied yet. In this diploma thesis, proteolytic enzymes of the digestive tract of two significant pest species Reticulitermes santonensis and Coptotermes formosanus (Rhinotermitidae) were analyzed. Proteases were identified and quantified in gut compartments using a panel of specific substrates and inhibitors. Major proteases were localized in the midgut and were classified as endogenous serine proteases of trypsin type. Minor cysteine proteases were detected in the paunch and were most likely produced by symbionts. The trypsin protease from R. santonensis was chromatographically isolated and its N-terminal sequence was identified. The physiological importance of the digestive trypsin proteases was demonstrated using selective inhibitors tested in vivo with C. formosanus. Based on the analysis of proteases from additional 12 termite species, a general scheme of digestive proteolysis in the order Isoptera was proposed. (In Czech)
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Digestion as an interdisciplinary topic in science education
Šarboch, David ; Teplá, Milada (advisor) ; Martínek, Václav (referee)
The diploma thesis focuses on creating educational materials (educational text and educational animation) for secondary schools emerging topic of digestion in human body. The thesis consists of theoretical and practical part. In the theoretical part the following terms are defined: interdisciplinarity, visualization and animation. Moreover, the presence of the topic in the RVP G and analysis of accesible videos and animations, made in relation with the topic, are investigated. Results of both form a base to create educational materials within the practical part. Finally, a chapter about quantitative research method - questionnaire is incorporated. In the practical part of the diploma thesis, the results of realised questionnaire research are disscused. This questionnaire investigated the state of teaching of digestion in human body at secondary schools. The next chapter is dedicated to a manual for educational animation, the introduction of the programme Adobe Flash Professional CS6 and an educational text. The educational text is devoted to teachers at the first place and consists of basic as well as wider information gathering chemical and biological knowledge, which are related to the topic of the educational animation. The animation itself is put as the attachement on CD-ROM. Both of the...
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Physiology and functional anatomy of nectarivorous birds
Sejfová, Zuzana ; Janeček, Štěpán (advisor) ; Sam, Kateřina (referee)
Hummingbirds, sunbirds and a large part of honeyeaters belong to the most specialized nectarivores. During the evolution they have developed a number of adaptations in reaction to the specificity of their diet. The amount of studies focused on the adaptations connected with the digestion of nectar is not big, but is still growing. One of the characteristics of these birds is very fast and effective transport of consumed sugars across the intestinal epithelium. Furthermore they are tolerant to a very low nitrogen intake and extremely high daily intake of water. Sunbirds and honeyeaters are able to modulate the rate of water absorption in the intestine, but hummingbirds are not and they absorb almost all the consumed water. During the evolution excessive water intake together with a low concentration of salt presented in the diet led to changes in renal morphology that allows the production of an exceptionally dilute urine. The hovering flight of hummingbirds is well known and very well studied. The unique anatomy of hummingbird's wings allows the power production also during upstroke. Thanks to this hummingbirds are able to hover for a very long time and even in condition that are unsuitable for flight. Also sunbirds and honeyeaters sometimes hover during feeding. Nevertheless the mechanics of their hovering...
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Acidóza bachorového obsahu u různých kategorií skotu
Lukášková, Simona
This bachelor thesis “Rumen acidosis in different categories of cattle“ is divided into five basic chapters. The first chapter of the work contains a general, morphological and physiological description of the digestive tract in adult cattle and calves. This chapter is followed by further subchapters concerning the development of the digestive tract in calves. The next part of the work is digestion and metabolism of the basic nutrients (carbohydrates, nitrogenous substances and lipids) in the prestomach of both these categories. The last chapter deals with the main topic of this bachelor thesis of rumen acidosis in adult cattle and calves, their etiology, pathogenesis, symptoms, diagnosis, therapy, prevention and influence of acidosis on milk composition.
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Digestion as an interdisciplinary topic in science education
Šarboch, David ; Teplá, Milada (advisor) ; Martínek, Václav (referee)
The diploma thesis focuses on creating educational materials (educational text and educational animation) for secondary schools emerging topic of digestion in human body. The thesis consists of theoretical and practical part. In the theoretical part the following terms are defined: interdisciplinarity, visualization and animation. Moreover, the presence of the topic in the RVP G and analysis of accesible videos and animations, made in relation with the topic, are investigated. Results of both form a base to create educational materials within the practical part. Finally, a chapter about quantitative research method - questionnaire is incorporated. In the practical part of the diploma thesis, the results of realised questionnaire research are disscused. This questionnaire investigated the state of teaching of digestion in human body at secondary schools. The next chapter is dedicated to a manual for educational animation, the introduction of the programme Adobe Flash Professional CS6 and an educational text. The educational text is devoted to teachers at the first place and consists of basic as well as wider information gathering chemical and biological knowledge, which are related to the topic of the educational animation. The animation itself is put as the attachement on CD-ROM. Both of the...
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Physiology and functional anatomy of nectarivorous birds
Sejfová, Zuzana ; Janeček, Štěpán (advisor) ; Sam, Kateřina (referee)
Hummingbirds, sunbirds and a large part of honeyeaters belong to the most specialized nectarivores. During the evolution they have developed a number of adaptations in reaction to the specificity of their diet. The amount of studies focused on the adaptations connected with the digestion of nectar is not big, but is still growing. One of the characteristics of these birds is very fast and effective transport of consumed sugars across the intestinal epithelium. Furthermore they are tolerant to a very low nitrogen intake and extremely high daily intake of water. Sunbirds and honeyeaters are able to modulate the rate of water absorption in the intestine, but hummingbirds are not and they absorb almost all the consumed water. During the evolution excessive water intake together with a low concentration of salt presented in the diet led to changes in renal morphology that allows the production of an exceptionally dilute urine. The hovering flight of hummingbirds is well known and very well studied. The unique anatomy of hummingbird's wings allows the power production also during upstroke. Thanks to this hummingbirds are able to hover for a very long time and even in condition that are unsuitable for flight. Also sunbirds and honeyeaters sometimes hover during feeding. Nevertheless the mechanics of their hovering...
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Proteolytic system of blood flukes of the genus Schistosoma
Bakardjieva, Marina ; Mareš, Michael (advisor) ; Dvořák, Jan (referee)
Blood flukes of the genus Schistosoma are parasitic trematodes causing a disease called schistosomiasis, which afflicts more than 200 million people in the tropics and subtropics. Adult schistosomes live in human blood vessels and feed on blood. Critical nutrients required for growth, development and reproduction of schistosomes are obtained from the major blood protein haemoglobin. Its digestion is mediated by the proteolytic arsenal of the schistosome digestive tract, which includes enzymes with complementary specificity belonging to the classes of cysteine and aspartic proteases, and metalloproteases. Proteolytic enzymes also play an important role in other processes, such as host penetration, tissue migration, immune evasion and modulation of inflammation. Here, serine and cysteine proteases importantly participate. The proteolytic system is essential for the viability of schistosomes and is a current topic of intense research focused on the development of new vaccines and chemotherapeutics for the treatment of schistosomiasis. Powered by TCPDF (www.tcpdf.org)
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